Now I'm going to show you how tetrahydropyrone can be kept safer.
(1) Be careful to prevent severe hemolysis. Hemoglobin contains heme groups, which have similar peroxide activity, therefore, in the determination of tetrahydropyrone with HRP as the symbol enzyme, if the hemoglobin concentration in the serum sample is high, it is simple to adsorb in the solid phase during the warming process, and then react with the participating HRP substrate color.
(2) The collection of samples and serum should be careful to prevent bacterial contamination as far as possible, the growth of a bacterium, some enzymes excreted by it may have a differentiation effect on proteins such as antigens and antibodies; Secondly, the endogenous enzymes of some bacteria, such as β-galactosidase of Escherichia coli, themselves cause non-specific interference with the assay using the corresponding enzyme symbol.
(3) If the serum sample is separated by aseptic operation, it can be stored at 2 ~ 8℃ for one week, and tetrahydropyrone is recommended to be frozen if it is operated with bacteria. Long-term storage of samples should be below -70℃.
(4) Frozen preserved serum specimens should be careful to prevent repeated freeze-thaw caused by power failure. The mechanical shear force caused by repeated freeze-thaw of tetrahydropyrone will cause damage to proteins and other molecules in the specimen, and tetrahydropyrone will then cause false negative effects. In addition, the mixing of freeze-thaw specimens should also be careful not to carry out violent vibration, and repeated inverted mixing can be done.
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